A Thin Film Characterization Technique
In vitro results showed that UV–C treatment not only improved the cell spreading and attachment, but also it made the cells more metabolically active. UV–C effectively removed the hydrocarbon contaminants and exposed the TiO2 on the surface, which is a good indication for higher and faster bone–to–implant contact than hydrocarbon–coated implants. Larsson et al. (1996) showed that high degrees of bone contact and bone formation were achieved with titanium implants which were modified on oxide thickness. On the other hand, Aita et al. (2009) revealed that UV–induced biological effects could be obtained even on the surfaces of titanium dioxide bulks without depositing additional oxidative titanium or sintering.
Ellipsometry, as a thin film characterization technique, was selected because it is non–invasive, non–destructive, non–contacting, and can allow the user to determine several film properties simultaneously (Woollam JA, Snyder PG 1990; Vedam K 1998). Based on this, the tested samples did not undergo any surface modifications by this device. Also, all the work for the crucial thickness measurements (ellipsometry) and the UV–C illumination (light source) was done in a HEPA–filter–protected Clean Room. The total number of particles available for adsorption (in the background) were fewer than 100 condensation nuclei / cc, which met clean room standards for NASA class–100 (Clean Room Fact Sheet, NASA 2016). The measured refractive index (R.I) for the baseline commercially
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